Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

Abstract

Summary: Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).