Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background

Ying Wang; Hongxiang Yu; Yue Zhang; Jing Zhang; Keni Chen; Heng Sun; Shulin Meng; Gang Li; Ying Lei; Jian Zhao

Stem Cell Research (Mar 2018)

Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background

Ying Wang,
Hongxiang Yu,
Yue Zhang,
Jing Zhang,
Keni Chen,
Heng Sun,
Shulin Meng,
Gang Li,
Ying Lei,
Jian Zhao

Affiliations

Ying Wang: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Hongxiang Yu: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Yue Zhang: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Jing Zhang: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Keni Chen: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Heng Sun: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Shulin Meng: IxCell Biotechnology Co., Ltd, Shanghai, China
Gang Li: Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding authors.
Ying Lei: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding authors.
Jian Zhao: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China

Journal volume & issue: Vol. 27
pp. 57 – 60

Abstract

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A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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