Nature Communications (Jun 2020)
Low-dose phase retrieval of biological specimens using cryo-electron ptychography
- Liqi Zhou,
- Jingdong Song,
- Judy S. Kim,
- Xudong Pei,
- Chen Huang,
- Mark Boyce,
- Luiza Mendonça,
- Daniel Clare,
- Alistair Siebert,
- Christopher S. Allen,
- Emanuela Liberti,
- David Stuart,
- Xiaoqing Pan,
- Peter D. Nellist,
- Peijun Zhang,
- Angus I. Kirkland,
- Peng Wang
Affiliations
- Liqi Zhou
- National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences and Collaborative Innovation Center of Advanced Microstructures, Nanjing University
- Jingdong Song
- State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention
- Judy S. Kim
- Department of Materials, University of Oxford, Parks Road
- Xudong Pei
- National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences and Collaborative Innovation Center of Advanced Microstructures, Nanjing University
- Chen Huang
- Department of Materials, University of Oxford, Parks Road
- Mark Boyce
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Luiza Mendonça
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Daniel Clare
- Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus
- Alistair Siebert
- Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus
- Christopher S. Allen
- Department of Materials, University of Oxford, Parks Road
- Emanuela Liberti
- Department of Materials, University of Oxford, Parks Road
- David Stuart
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Xiaoqing Pan
- Department of Materials Science and Engineering, and Department of Physics and Astronomy, University of California
- Peter D. Nellist
- Department of Materials, University of Oxford, Parks Road
- Peijun Zhang
- Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
- Angus I. Kirkland
- Department of Materials, University of Oxford, Parks Road
- Peng Wang
- National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences and Collaborative Innovation Center of Advanced Microstructures, Nanjing University
- DOI
- https://doi.org/10.1038/s41467-020-16391-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show that electron ptychography is more efficient for phase recovery than conventional phase contrast imaging.
