Terminal deoxynucleotidyl transferase: Properties and applications

Chengjie Zhang; Hizar Subthain; Fei Guo; Peng Fang; Shanmin Zheng; Mengzhe Shen; Xianger Jiang; Zhengquan Gao; Chunxiao Meng; Shengying Li; Lei Du

Engineering Microbiology (Mar 2025)

Terminal deoxynucleotidyl transferase: Properties and applications

Chengjie Zhang,
Hizar Subthain,
Fei Guo,
Peng Fang,
Shanmin Zheng,
Mengzhe Shen,
Xianger Jiang,
Zhengquan Gao,
Chunxiao Meng,
Shengying Li,
Lei Du

Affiliations

Chengjie Zhang: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China
Hizar Subthain: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China
Fei Guo: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China
Peng Fang: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China
Shanmin Zheng: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China; School of Pharmacy, Binzhou Medical University, Yantai, 264003, Shandong, China
Mengzhe Shen: BGI Research, Shenzhen, 518083, China
Xianger Jiang: BGI Research, Shenzhen, 518083, China
Zhengquan Gao: School of Pharmacy, Binzhou Medical University, Yantai, 264003, Shandong, China
Chunxiao Meng: School of Pharmacy, Binzhou Medical University, Yantai, 264003, Shandong, China; Corresponding authors.
Shengying Li: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, Shandong, China; Corresponding authors.
Lei Du: State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China; Corresponding authors.

Journal volume & issue: Vol. 5, no. 1
p. 100179

Abstract

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Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable DNA synthesis activity, TdTs have promoted the development of numerous nucleic acid-based methods, tools, and associated applications, attracting broad interest from both academia and industry. This review summarizes and discusses the recent research on TdTs, including their biochemical characteristics, enzyme engineering, and practical applications. New insights and perspectives on the future development of TdTs are provided.

Published in Engineering Microbiology

ISSN: 2667-3703 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: https://www.sciencedirect.com/journal/engineering-microbiology

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Abstract

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