Elution of proteins from polyacrylamide eels: a simple and economic procedure

Abstract

A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure developed for use in conventional tube gel electrophoresis equipment.

Keywords

• Poliacrilamida
• Eletroeluição
• Proteínas