Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides

XIE Hui; LIU An; CHEN Liang; WU Xiaofen; QI Hui; ZHANG Qiling; WANG Zhen; DENG Ming; WANG Keqin; LIU Suchun

doi:10.11889/j.1000-3436.2023-0029

Fushe yanjiu yu fushe gongyi xuebao (Jun 2023)

Radiation mutagenesis of Bacillus velezensis and its antibacterial effect on Colletotrichum gloeosporioides

XIE Hui,
LIU An,
CHEN Liang,
WU Xiaofen,
QI Hui,
ZHANG Qiling,
WANG Zhen,
DENG Ming,
WANG Keqin,
LIU Suchun

Affiliations

XIE Hui: College of Food Science and Technology, Hunan Agricultural University, Changsha 410125, China
LIU An: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
CHEN Liang: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
WU Xiaofen: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
QI Hui: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
ZHANG Qiling: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
WANG Zhen: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
DENG Ming: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
WANG Keqin: Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
LIU Suchun: College of Food Science and Technology, Hunan Agricultural University, Changsha 410125, China

DOI: https://doi.org/10.11889/j.1000-3436.2023-0029
Journal volume & issue: Vol. 41, no. 3
pp. 030401 – 030401

Abstract

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We used 60Co-γ ray and electron beam irradiation to mutate Bacillus velezensis to breed genetically stable mutant strains. The mutant strains were screened and re-screened by plate confrontation and the four-point method to study their antibacterial effects on Colletotrichum gloeosporioides. The results demonstrated that the D10 value of Bacillus velezensis suspension irradiated by 60Co-γ ray was 2 366 Gy, and the lethality rate increased with an increase in the irradiation coefficient dose in the range of 100-2 000 Gy. The lethality rate of Bacillus velezensis was 81.8% at 2 000 Gy irradiation coefficient dose, and five mutants (B004, B112, B114, B117, and B118) were screened. The inhibition rate against Colletotrichum gloeosporioides increased from 51.2% of the original strain to 57.5%, 58.0%, 57.0%, 57.7%, and 59.9%, respectively. The Bacillus velezensis suspension was irradiated by an electron beam (10 MeV), the D10 value was 499 Gy, and the lethality rate increased with an increase in the irradiation coefficient dose in the range of 100-600 Gy. The lethality rate of Bacillus velezensis was 91.8% at 400 Gy irradiation coefficient dose. Two mutant strains (D115 and D243) were screened, and their inhibition rates against Colletotrichum gloeosporioides increased from 55.2% of the emitted strains to 58.4 and 58.1%, respectively. The inhibitory effects of B004, B112, B117, and B118 mutants on Colletotrichum gloeosporioides were genetically stable within six generations. In conclusion, γ-ray and electron beam irradiation have potential application value in the mutation breeding of Bacillus velezensis. The results provide a basis for Bacillus velezensis mutation breeding and a theoretical reference for the biological control of Colletotrichum gloeosporioides.

Published in Fushe yanjiu yu fushe gongyi xuebao

ISSN: 1000-3436 (Print)
Publisher: Science Press
Country of publisher: China
LCC subjects: Science: Physics: Nuclear and particle physics. Atomic energy. Radioactivity; Medicine: Medicine (General): Medical physics. Medical radiology. Nuclear medicine
Website: https://www.fs.sinap.ac.cn/

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