The formation of carnation plants in Callus derived from the cultivation of cellular pods by way of landfill in the multiple droplets

Abstract

ABSTRACT Stem callus cultures of carnation (Dianthus caryophyllus) local variety were grown on asynthetic medium Murashige and Skoog (MS) medium. Optimal concentration of growth regulators were observed to be 0.5mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0.1mg/L Benzyle adenine (BA). Cell suspenion cultures worked best in liquid MS medium suspplemented with 0.5mg/L 2,4-D and 0.1mg/L BA successfully plated in agar drops is petridishes. Cell divisions led to the callus formation. Successful shoot regeneration occurred in agar solidified MS medium containing 0.06mg/L indole- acetic acid (IAA) and 1.0 mg/L Benzyle adenin (BA). Shoot were easily rooted and transferred to soil.

Keywords

• carnation plants
• cultivation
• multiple droplets