Frontiers in Immunology (Jan 2023)
Identification of PDCD1 as a potential biomarker in acute rejection after kidney transplantation via comprehensive bioinformatic analysis
- Yucheng Wang,
- Yucheng Wang,
- Yucheng Wang,
- Yucheng Wang,
- Xiaoli Lin,
- Xiaoli Lin,
- Xiaoli Lin,
- Xiaoli Lin,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Cuili Wang,
- Xinyu Liu,
- Xinyu Liu,
- Xinyu Liu,
- Xinyu Liu,
- Xiaoying Wu,
- Xiaoying Wu,
- Xiaoying Wu,
- Xiaoying Wu,
- Yingying Qiu,
- Yingying Qiu,
- Yingying Qiu,
- Yingying Qiu,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Qin Zhou,
- Qin Zhou,
- Qin Zhou,
- Qin Zhou,
- Haige Zhao,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Jianghua Chen,
- Hongfeng Huang,
- Hongfeng Huang,
- Hongfeng Huang,
- Hongfeng Huang
Affiliations
- Yucheng Wang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Yucheng Wang
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Yucheng Wang
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Yucheng Wang
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Xiaoli Lin
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Xiaoli Lin
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Xiaoli Lin
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Xiaoli Lin
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Cuili Wang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Cuili Wang
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Cuili Wang
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Cuili Wang
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Xinyu Liu
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Xinyu Liu
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Xinyu Liu
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Xinyu Liu
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Xiaoying Wu
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Xiaoying Wu
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Xiaoying Wu
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Xiaoying Wu
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Yingying Qiu
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Yingying Qiu
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Yingying Qiu
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Yingying Qiu
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Ying Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Ying Chen
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Ying Chen
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Ying Chen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Qin Zhou
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Qin Zhou
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Qin Zhou
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Qin Zhou
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Haige Zhao
- Department of Cardiothoracic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
- Jianghua Chen
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Jianghua Chen
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Jianghua Chen
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Jianghua Chen
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- Hongfeng Huang
- Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
- Hongfeng Huang
- Key Laboratory of Kidney Disease Prevention and Control Technology, Hangzhou, Zhejiang, China
- Hongfeng Huang
- Institute of Nephrology, Zhejiang University, Hangzhou, Zhejiang, China
- Hongfeng Huang
- Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Zhejiang, China
- DOI
- https://doi.org/10.3389/fimmu.2022.1076546
- Journal volume & issue
-
Vol. 13
Abstract
BackgroundAcute rejection is a determinant of prognosis following kidney transplantation. It is essential to search for novel noninvasive biomarkers for early diagnosis and prompt treatment.MethodsGene microarray data was downloaded from the Gene Expression Omnibus (GEO) expression profile database and the intersected differentially expressed genes (DEGs) was calculated. We conducted the DEGs with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Distribution of immune cell infiltration was calculated by CIBERSORT. A hub gene marker was identified by intersecting the rejection-related genes from WGCNA and a selected KEGG pathway—T cell receptor signaling pathway (hsa04660), and building a protein-protein interaction network using the STRING database and Cytoscape software. We performed flow-cytometry analysis to validate the hub gene.ResultsA total of 1450 integrated DEGs were obtained from five datasets (GSE1563, GSE174020, GSE98320, GSE36059, GSE25902). The GO, KEGG and immune infiltration analysis results showed that AR was mainly associated with T cell activation and various T-cell related pathways. Other immune cells, such as B cells, Macrophage and Dendritic cells were also associated with the progress. After utilizing the WGCNA and PPI network, PDCD1 was identified as the hub gene. The flow-cytometry analysis demonstrated that both in CD4+ and CD8+ T cells, PD1+CD57-, an exhausted T cell phenotype, were downregulated in the acute rejection whole blood samples.ConclusionsOur study illustrated that PDCD1 may be a candidate diagnostic biomarker for acute kidney transplant rejection via integrative bioinformatic analysis.
Keywords
- acute rejection
- PDCD1
- kidney transplantation
- biomarker
- immune infiltration
- weighted gene co-expression network analysis
