Cell Reports (Oct 2023)
ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells
- M. Carmen Lafita-Navarro,
- Yi-Heng Hao,
- Chunhui Jiang,
- Seoyeon Jang,
- Tsung-Cheng Chang,
- Isabella N. Brown,
- Niranjan Venkateswaran,
- Elizabeth Maurais,
- Weronika Stachera,
- Yanfeng Zhang,
- Dorothy Mundy,
- Jungsoo Han,
- Vanna M. Tran,
- Marcel Mettlen,
- Lin Xu,
- Jeffrey B. Woodruff,
- Nick V. Grishin,
- Lisa Kinch,
- Joshua T. Mendell,
- Michael Buszczak,
- Maralice Conacci-Sorrell
Affiliations
- M. Carmen Lafita-Navarro
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Yi-Heng Hao
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Chunhui Jiang
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Seoyeon Jang
- Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Tsung-Cheng Chang
- Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Isabella N. Brown
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Niranjan Venkateswaran
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Elizabeth Maurais
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Weronika Stachera
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Yanfeng Zhang
- Quantitative Biomedical Research Center, Department of Population & Data Sciences, Peter O’Donnell Jr. School of Public Health, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Dorothy Mundy
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Live Cell Imaging Core Facility, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Jungsoo Han
- Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Vanna M. Tran
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Marcel Mettlen
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Lin Xu
- Quantitative Biomedical Research Center, Department of Population & Data Sciences, Peter O’Donnell Jr. School of Public Health, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Jeffrey B. Woodruff
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Nick V. Grishin
- Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Lisa Kinch
- Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Joshua T. Mendell
- Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Michael Buszczak
- Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
- Maralice Conacci-Sorrell
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Corresponding author
- Journal volume & issue
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Vol. 42,
no. 10
p. 113280
Abstract
Summary: Increased nucleolar size and activity correlate with aberrant ribosome biogenesis and enhanced translation in cancer cells. One of the first and rate-limiting steps in translation is the interaction of the 40S small ribosome subunit with mRNAs. Here, we report the identification of the zinc finger protein 692 (ZNF692), a MYC-induced nucleolar scaffold that coordinates the final steps in the biogenesis of the small ribosome subunit. ZNF692 forms a hub containing the exosome complex and ribosome biogenesis factors specialized in the final steps of 18S rRNA processing and 40S ribosome maturation in the granular component of the nucleolus. Highly proliferative cells are more reliant on ZNF692 than normal cells; thus, we conclude that effective production of small ribosome subunits is critical for translation efficiency in cancer cells.
