Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression
Dou Ye,
Suqing Qu,
Yinxiang Yang,
Zhaoyan Wang,
Qian Wang,
Weipeng Liu,
Fan Zhang,
Qian Guan,
Xiaohua Wang,
Jing Zang,
Xin Li,
Hengtao Liu,
Ruiqin Yao,
Zhichun Feng,
Zuo Luan
Affiliations
Dou Ye
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China; Medical School of Chinese PLA, Beijing 100853, China
Suqing Qu
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Yinxiang Yang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Zhaoyan Wang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Qian Wang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Weipeng Liu
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Fan Zhang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China; Medical School of Chinese PLA, Beijing 100853, China
Qian Guan
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Xiaohua Wang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Jing Zang
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Xin Li
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China
Hengtao Liu
Jiaen Genetics Laboratory, Beijing Jiaen Hospital, Beijing 100191, China
Ruiqin Yao
Department of Neurobiology, Xuzhou Key Laboratory of Neurobiology, Xuzhou Medical University, Xuzhou, Jiangsu Province 221004, China; Corresponding author
Zhichun Feng
Faculty of Pediatrics, The Seventh Medical Centre, Chinese PLA General Hospital, 100700 Beijing, China; Corresponding author
Zuo Luan
Department of Pediatrics, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing 100037, China; Medical School of Chinese PLA, Beijing 100853, China; Corresponding author
Summary: Immune rejection can be reduced using immunosuppressants which are not viable for premature infants. However, desensitization can induce immune tolerance for premature infants because of underdeveloped immune system. The fetuses of Wistar rats at 15–17 days gestation were injected via hOPCs-1 into brain, muscles, and abdomen ex utero and then returned while the fetuses of control without injection. After 6 weeks of desensitization, the brain and muscles were transplanted with hOPCs-1, hNSCs-1, and hOPCs-2. After 10 and 34 weeks of desensitization, hOPCs-1 and hNSCs-1 in desensitized groups was higher than that in the control group while hOPCs-2 were rejected. Treg, CD4CD28, CD8CD28, and CD45RC between the desensitization and the control group differed significantly. Inflammatory cells in group with hOPCs-1 and hNSCs-1 was lower than that in the control group. hOPCs-1 can differentiate into myelin in desensitized groups. Wistar rats with desensitization developed immune tolerance to desensitized and transplanted cells.
