Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

Zhichen Xu; Dongjuan Chen; Tao Li; Jiayu Yan; Jiang Zhu; Ting He; Rui Hu; Ying Li; Yunhuang Yang; Maili Liu

doi:10.1038/s41467-022-34086-y

Nature Communications (Oct 2022)

Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

Zhichen Xu,
Dongjuan Chen,
Tao Li,
Jiayu Yan,
Jiang Zhu,
Ting He,
Rui Hu,
Ying Li,
Yunhuang Yang,
Maili Liu

Affiliations

Zhichen Xu: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Dongjuan Chen: Department of Laboratory Medicine, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology
Tao Li: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Jiayu Yan: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Jiang Zhu: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Ting He: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Rui Hu: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Ying Li: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Yunhuang Yang: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences
Maili Liu: State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology—Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-022-34086-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Fast, low-cost and multiplexed nucleic acid detection is challenging. Here the authors report a strategy that couples microfluidic space coding, CRISPRCas12a, and multiplex RPA for the rapid detection of up to 30 targets with only one fluorescent probe.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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