TESTING OF TWO PROTOCOLS FOR GENOTYPING THE LEPTIN GENE LOCUS AND BODY MEASUREMENT IN MARAMURES BROWN AND ROMANIAN BREED CATTLE

Abstract

The body measurement of Maramures Brown breed and Romanian Siemmental and testing two protocols for emphasizing the leptine gene in order to perform associations with some beef production traits within further studies were the aims of our research. The blood DNA extraction was performed according to the protocols proposed by Yves Amigues and genotyping protocols was proposed by Leifers and Pomp et al. The body weight is within standards. The analyzed protocols used for leptine gene emphasizing led to satisfactory results, which will enable us to perform further research in order to make associations between this possible marker gene and some body traits.

Keywords

• leptine
• cattle
• traits
• meat