Supporting the Wound Healing Process—Curcumin, Resveratrol and Baicalin in In Vitro Wound Healing Studies

Kacper Jagiełło; Oliwia Uchańska; Konrad Matyja; Mateusz Jackowski; Benita Wiatrak; Paweł Kubasiewicz-Ross; Ewa Karuga-Kuźniewska

doi:10.3390/ph16010082

Pharmaceuticals (Jan 2023)

Supporting the Wound Healing Process—Curcumin, Resveratrol and Baicalin in In Vitro Wound Healing Studies

Kacper Jagiełło,
Oliwia Uchańska,
Konrad Matyja,
Mateusz Jackowski,
Benita Wiatrak,
Paweł Kubasiewicz-Ross,
Ewa Karuga-Kuźniewska

Affiliations

Kacper Jagiełło: Department of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wroclaw University of Science and Technology, ul. Gdańska 7/9, 50-344 Wrocław, Poland
Oliwia Uchańska: Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366 Wrocław, Poland
Konrad Matyja: Department of Micro, Nano, and Bioprocess Engineering, Faculty of Chemistry, Wroclaw University of Science and Technology, ul. Norwida 4/6, 50-373 Wrocław, Poland
Mateusz Jackowski: Department of Micro, Nano, and Bioprocess Engineering, Faculty of Chemistry, Wroclaw University of Science and Technology, ul. Norwida 4/6, 50-373 Wrocław, Poland
Benita Wiatrak: Department of Pharmacology, Wroclaw Medical University, ul. Jana Mikulicza-Radeckiego 2, 50-345 Wrocław, Poland
Paweł Kubasiewicz-Ross: Department and Clinic of Dental Surgery, Wroclaw Medical University, ul. Krakowska 26, 50-425 Wrocław, Poland
Ewa Karuga-Kuźniewska: Department of Epizootiology with Exotic Animal and Bird Clinic, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wrocław, Poland

DOI: https://doi.org/10.3390/ph16010082
Journal volume & issue: Vol. 16, no. 1
p. 82

Abstract

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The purpose of the investigation was to evaluate the effect of the selected bioflavonoids curcumin, resveratrol and baicalin on the wound healing process in an in vitro model. In the study, Balb3t3 and L929 cell lines were used. The first step was the evaluation of the cytotoxicity of the substances tested (MTT assay). Then, using the scratch test (ST), the influence of bioflavonoids on the healing process was evaluated in an in vitro model. The second stage of the work was a mathematical analysis of the results obtained. On the basis of experimental data, the parameters of the Brian and Cousens model were determined in order to determine the maximum value of the cellular and metabolic response that occurs for the examined range of concentrations of selected bioflavonoids. In the MTT assays, no cytotoxic effect of curcumin, resveratrol and baicalin was observed in selected concentrations, while in the ST tests for selected substances, a stimulatory effect was observed on the cell division rate regardless of the cell lines tested. The results obtained encourage further research on the use of substances of natural origin to support the wound healing process.

Published in Pharmaceuticals

ISSN: 1424-8247 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceuticals/

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