Viruses (May 2023)
Novel Amplicon-Based Sequencing Approach to West Nile Virus
- Moussa Moïse Diagne,
- Marie Henriette Dior Ndione,
- Giulia Mencattelli,
- Amadou Diallo,
- El hadji Ndiaye,
- Marco Di Domenico,
- Diawo Diallo,
- Mouhamed Kane,
- Valentina Curini,
- Ndeye Marieme Top,
- Maurilia Marcacci,
- Maïmouna Mbanne,
- Massimo Ancora,
- Barbara Secondini,
- Valeria Di Lollo,
- Liana Teodori,
- Alessandra Leone,
- Ilaria Puglia,
- Alioune Gaye,
- Amadou Alpha Sall,
- Cheikh Loucoubar,
- Roberto Rosà,
- Mawlouth Diallo,
- Federica Monaco,
- Ousmane Faye,
- Cesare Cammà,
- Annapaola Rizzoli,
- Giovanni Savini,
- Oumar Faye
Affiliations
- Moussa Moïse Diagne
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Marie Henriette Dior Ndione
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Giulia Mencattelli
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Amadou Diallo
- Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- El hadji Ndiaye
- Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Marco Di Domenico
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Diawo Diallo
- Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Mouhamed Kane
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Valentina Curini
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Ndeye Marieme Top
- Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Maurilia Marcacci
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Maïmouna Mbanne
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Massimo Ancora
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Barbara Secondini
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Valeria Di Lollo
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Liana Teodori
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Alessandra Leone
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Ilaria Puglia
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Alioune Gaye
- Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Amadou Alpha Sall
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Cheikh Loucoubar
- Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Roberto Rosà
- Centre Agriculture Food Environment, University of Trento, 38010 San Michele all’Adige, Italy
- Mawlouth Diallo
- Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Federica Monaco
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Ousmane Faye
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- Cesare Cammà
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Annapaola Rizzoli
- Research and Innovation Centre, Fondazione Edmund Mach, 38010 San Michele all’Adige, Italy
- Giovanni Savini
- Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, 64100 Teramo, Italy
- Oumar Faye
- Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal
- DOI
- https://doi.org/10.3390/v15061261
- Journal volume & issue
-
Vol. 15,
no. 6
p. 1261
Abstract
West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel whole-genome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.
Keywords
