Annals of Clinical Microbiology and Antimicrobials (Jan 2010)

Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria

  • Budak Fatma,
  • Kolayli Fetiye,
  • Torol Sinem,
  • Fashae Kayode,
  • Kasap Murat,
  • Vahaboglu Haluk

DOI
https://doi.org/10.1186/1476-0711-9-1
Journal volume & issue
Vol. 9, no. 1
p. 1

Abstract

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Abstract Background We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer. Methods Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments. Results By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26. Conclusion This is the first study demonstrated the occurrence of SHV-12 in Nigeria.