Frontiers in Physiology (Oct 2024)
Nonlinear pathological trajectory of a high-myopia C57/BL6J mouse model induced by form deprivation
- Yue Wen,
- Yue Wen,
- Yue Wen,
- Yue Wen,
- Yan Li,
- Yan Li,
- Yan Li,
- Yan Li,
- Li Zhu,
- Li Zhu,
- Li Zhu,
- Li Zhu,
- Tao Tang,
- Tao Tang,
- Tao Tang,
- Tao Tang,
- Huichao Yan,
- Huichao Yan,
- Huichao Yan,
- Huichao Yan,
- Jie Hu,
- Jie Hu,
- Jie Hu,
- Jie Hu,
- Kai Wang,
- Kai Wang,
- Kai Wang,
- Kai Wang,
- Mingwei Zhao,
- Mingwei Zhao,
- Mingwei Zhao,
- Mingwei Zhao,
- Qiong Xu,
- Qiong Xu,
- Qiong Xu,
- Qiong Xu
Affiliations
- Yue Wen
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Yue Wen
- College of Optometry, Peking University Health Science Center, Beijing, China
- Yue Wen
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Yue Wen
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Yan Li
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Yan Li
- College of Optometry, Peking University Health Science Center, Beijing, China
- Yan Li
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Yan Li
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Li Zhu
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Li Zhu
- College of Optometry, Peking University Health Science Center, Beijing, China
- Li Zhu
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Li Zhu
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Tao Tang
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Tao Tang
- College of Optometry, Peking University Health Science Center, Beijing, China
- Tao Tang
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Tao Tang
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Huichao Yan
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Huichao Yan
- College of Optometry, Peking University Health Science Center, Beijing, China
- Huichao Yan
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Huichao Yan
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Jie Hu
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Jie Hu
- College of Optometry, Peking University Health Science Center, Beijing, China
- Jie Hu
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Jie Hu
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Kai Wang
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Kai Wang
- College of Optometry, Peking University Health Science Center, Beijing, China
- Kai Wang
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Kai Wang
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Mingwei Zhao
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Mingwei Zhao
- College of Optometry, Peking University Health Science Center, Beijing, China
- Mingwei Zhao
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Mingwei Zhao
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- Qiong Xu
- Department of Ophthalmology and Clinical Center of Optometry, Peking University People’s Hospital, Beijing, China
- Qiong Xu
- College of Optometry, Peking University Health Science Center, Beijing, China
- Qiong Xu
- Eye Disease and Optometry Institute, Peking University People’s Hospital, Beijing, China
- Qiong Xu
- Beijing Key Laboratory of Diagnosis and Therapy of Retinal and ChoroidDiseases, Beijing, China
- DOI
- https://doi.org/10.3389/fphys.2024.1442000
- Journal volume & issue
-
Vol. 15
Abstract
IntroductionTo establish a high myopia model in C57BL/6J mice with monocular form deprivation myopia (FDM) and investigate its ocular structure pathological trajectory.MethodsHealthy 3-week-old C57BL/6J mice were divided into an FDM group (n = 36) and a control group (n = 24). The left eyes of the FDM group were patched, while the right eyes served as controls. Biometric parameters and fundus morphology were assessed at baseline and after 4, 8, and 12 weeks of form deprivation.ResultsSignificant differences were observed in the deprived eyes, including longer axial length, higher refractive power, deeper vitreous chambers, thinner retina, choroid, and sclera, and smaller scleral fibers’ diameters under a transmission electron microscope. Retinal vascular area proportion in covered eyes decreased significantly (P < 0.05), with a decline rate of 11% from weeks 4 to 8 and a faster decline of 19% from weeks 8 to 12, while this proportion increased significantly in control eyes.DiscussionThis study successfully induced a high myopia model in mice with long-term form deprivation. The axial length grew dramatically in FDM in the first 8 weeks, while the pathological progress of the fundus accelerated from weeks 8 to 12.
Keywords
- form deprivation myopia
- high myopia
- retinal vessel
- choroidal thickness
- scleral fiber
- nonlinear pathological trajectory
