Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25

Asha S. Salunke; Arun S. Kharat

Data in Brief (Oct 2019)

Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25

Asha S. Salunke,
Arun S. Kharat

Affiliations

Asha S. Salunke: Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Subcampus Osmanabad, 413501, Maharashtra, India; BioEra Life Sciences Pvt. Ltd., Survey Number 125, Mumbai – Bangalore Highway, Tathawade, Pune, 411033, Maharashtra, India
Arun S. Kharat: Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Subcampus Osmanabad, 413501, Maharashtra, India; Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India; Corresponding author. Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Subcampus Osmanabad, 413501, Maharashtra, India.

Journal volume & issue: Vol. 26

Abstract

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The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source. The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extraction and purification of fibrinolytic enzyme, (4) protein estimation, (5) assay of fibrinolytic activity, (6) SDS PAGE for purified enzyme protein, (7) effect of pH, temperature and metal ions on fibrinolytic activity of enzyme protein, and (8) In-vitro blood clot dissolution assay. Keywords: Pseudomonas baetica SUHU25, Fibrinolytic, Blood clot, Enzyme, Cardiovascular disease

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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