Nature Communications (Jan 2025)
A diagnostic host-specific transcriptome response for Mycoplasma pneumoniae pneumonia to guide pediatric patient treatment
- Sandra Viz-Lasheras,
- Alberto Gómez-Carballa,
- Xabier Bello,
- Irene Rivero-Calle,
- Ana Isabel Dacosta,
- Myrsini Kaforou,
- Dominic Habgood-Coote,
- Aubrey J. Cunnington,
- Marieke Emonts,
- Jethro A. Herberg,
- Victoria J. Wright,
- Enitan D. Carrol,
- Stephane C. Paulus,
- Werner Zenz,
- Daniela S. Kohlfürst,
- Nina Schweintzger,
- Michiel Van der Flier,
- Ronald de Groot,
- Luregn J. Schlapbach,
- Philipp Agyeman,
- Andrew J. Pollard,
- Colin Fink,
- Taco T. Kuijpers,
- Suzanne Anderson,
- Ulrich Von Both,
- Marko Pokorn,
- Dace Zavadska,
- María Tsolia,
- Henriëtte A. Moll,
- Clementien Vermont,
- Michael Levin,
- Federico Martinón-Torres,
- Antonio Salas,
- On behalf of EUCLIDS, PERFORM, and DIAMONDS consortia
Affiliations
- Sandra Viz-Lasheras
- Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela
- Alberto Gómez-Carballa
- Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela
- Xabier Bello
- Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela
- Irene Rivero-Calle
- Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)
- Ana Isabel Dacosta
- Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)
- Myrsini Kaforou
- Department of Infectious Disease, Imperial College London
- Dominic Habgood-Coote
- Department of Infectious Disease, Imperial College London
- Aubrey J. Cunnington
- Department of Infectious Disease, Imperial College London
- Marieke Emonts
- Paediatric Immunology, Infectious Diseases & Allergy, Great North Children’s Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust
- Jethro A. Herberg
- Department of Infectious Disease, Imperial College London
- Victoria J. Wright
- Department of Infectious Disease, Imperial College London
- Enitan D. Carrol
- Department of Infectious Diseases, Alder Hey Children’s NHS Foundation Trust
- Stephane C. Paulus
- Department of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research Centre
- Werner Zenz
- Department of General Paediatrics, Medical University of Graz, Graz
- Daniela S. Kohlfürst
- Department of General Paediatrics, Medical University of Graz, Graz
- Nina Schweintzger
- Department of General Paediatrics, Medical University of Graz, Graz
- Michiel Van der Flier
- Pediatric Infectious Diseases and Immunology, Wilhelmina Children’s Hospital, University Medical Center Utrecht
- Ronald de Groot
- Pediatric Infectious Diseases and Immunology, Amalia Children’s Hospital, and Section Pediatric Infectious Diseases, Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
- Luregn J. Schlapbach
- Department of Intensive Care and Neonatology, and Children’s Research Center, University Children’s Hospital Zürich, University of Zürich
- Philipp Agyeman
- Department of Pediatrics, Inselspital, Bern University Hospital, University of Bern
- Andrew J. Pollard
- Department of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research Centre
- Colin Fink
- Micropathology Ltd, University of Warwick
- Taco T. Kuijpers
- Division of Pediatric Immunology, Rheumatology and Infectious diseases, Emma Children’s Hospital, Amsterdam Univiersyt Medical Center (Amsterdam UMC)
- Suzanne Anderson
- Medical Research Council Unit at the London School of Hygene & Tropical Medicine
- Ulrich Von Both
- Infectious Diseases, Department of Pediatrics, Dr von Hauner Children’s Hospital, University Hospital, LMU Munich
- Marko Pokorn
- Division of Paediatrics, University Medical Centre Ljubljana and Medical Faculty, University of Ljubljana
- Dace Zavadska
- Children’s Clinical University Hospital, Rīga Stradins University
- María Tsolia
- Second Department of Paediatrics, National and Kapodistrian University of Athens (NKUA), School of Medicine, Panagiotis & Aglaia, Kyriakou Children’s Hospital
- Henriëtte A. Moll
- Department of Paediatric Infectious Diseases and Immunology, Erasmus MC Sophia Children’s Hospital
- Clementien Vermont
- Department of Paediatric Infectious Diseases and Immunology, Erasmus MC Sophia Children’s Hospital
- Michael Levin
- Department of Infectious Disease, Imperial College London
- Federico Martinón-Torres
- Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago (IDIS), 15706 Hospital Clínico Universitario de Santiago (SERGAS)
- Antonio Salas
- Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela
- On behalf of EUCLIDS, PERFORM, and DIAMONDS consortia
- DOI
- https://doi.org/10.1038/s41467-025-55932-9
- Journal volume & issue
-
Vol. 16,
no. 1
pp. 1 – 13
Abstract
Abstract Mycoplasma pneumoniae causes atypical pneumonia in children and young adults. Its lack of a cell wall makes it resistant to beta-lactams, which are the first-line treatment for typical pneumonia. Current diagnostic tests are time-consuming and have low specificity, leading clinicians to administer empirical antibiotics. Using a LASSO regression simulation approach and blood microarray data from 107 children with pneumonia (including 30 M. pneumoniae) we identify eight different transcriptomic signatures, ranging from 3-10 transcripts, that differentiate mycoplasma pneumonia from other bacterial/viral pneumonias with high accuracy (AUC: 0.84–0.95). Additionally, we demonstrate that existing signatures for broadly distinguishing viral/bacterial infections and viral/bacterial pneumonias are ineffective in distinguishing M. pneumoniae from viral pneumonia. The new signatures are successfully validated in an independent RNAseq cohort of children with pneumonia, demonstrating their robustness. The high sensibility of these signatures presents a valuable opportunity to guide the treatment and management of M. pneumoniae pneumonia patients.
