Enterovirus 71 induces pyroptosis of human neuroblastoma SH-SY5Y cells through miR-146a/ CXCR4 axis
Hengzhong Guo,
Yangyang Zhu,
Yu Zou,
Chaozhi Li,
Ya Wang,
Gejing De,
Lili Lu
Affiliations
Hengzhong Guo
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China
Yangyang Zhu
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China
Yu Zou
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China
Chaozhi Li
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China
Ya Wang
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China
Gejing De
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongcheng District, 100700, Beijing, PR China; Corresponding author.
Lili Lu
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, PR China; Corresponding author.
Enterovirus 71 (EV71) is a predominant causative pathogen of hand-foot-and-mouth disease (HFMD) in children. Compared with other HFMD-associated viruses, EV71 tends to induce more severe neurological complications and even death. However, the detailed mechanism of EV71 causes nervous system disorder is still unclear. In this study, we found that EV71 induced the GSDMD/NLRP3-mediated pyroptosis of SH-SY5Y cells through up-regulated miR-146a. Through bioinformatic analysis, we identified C-X-C chemokine receptor type 4 (CXCR4) as the potential target of miR-146a. We noticed that the expression of CXCR4 was regulated by miR-146a during EV71 infection. Moreover, our results show that over-expression of CXCR4 attenuated EV71-induced pyroptosis of SY-SY5Y cells. These results reveal a previously unrecognized mechanism in which EV71 induces nervous system cells damage through regulating miR-146a/CXCR4 mediated pyroptosis.
