Опухоли женской репродуктивной системы (Jul 2014)

Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells

  • M. A. Taipov,
  • Z. N. Nikiforova,
  • O. M. Pavlova,
  • I. A. Kudryavtsev,
  • N. Ye. Arnotskaya,
  • V. Ye. Shevchenko

DOI
https://doi.org/10.17650/1994-4098-2013-0-1-2-6-12
Journal volume & issue
Vol. 0, no. 1-2
pp. 6 – 12

Abstract

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The expression of the SMAD2, SMAD4, SMAD7, and СOX-2 genes and the levels of Smad4, phosphorylated Smad2, and Smad7 proteins were analyzed in the human breast cancer (BC) cell lines MCF-7, BT-474, and ZR-75-1. The SMAD2 and SMAD4 genes were found to suppress COX-2 gene expression in the estrogen receptor-positive BC cells and to be linked to their metastatic potential. Smad4 and Smad2 apparently suppress COX-2 mRNA and protein synthesis and Smad7 is their antagonist and stimulates COX-2 formation. Smad2, Smad4, Smad7 proteins may be regarded as new potential targets for targeted BC therapy.

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