CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

Ida Höijer; Anastasia Emmanouilidou; Rebecka Östlund; Robin van Schendel; Selma Bozorgpana; Marcel Tijsterman; Lars Feuk; Ulf Gyllensten; Marcel den Hoed; Adam Ameur

doi:10.1038/s41467-022-28244-5

Nature Communications (Feb 2022)

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

Ida Höijer,
Anastasia Emmanouilidou,
Rebecka Östlund,
Robin van Schendel,
Selma Bozorgpana,
Marcel Tijsterman,
Lars Feuk,
Ulf Gyllensten,
Marcel den Hoed,
Adam Ameur

Affiliations

Ida Höijer: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Anastasia Emmanouilidou: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Rebecka Östlund: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Robin van Schendel: Department of Human Genetics, Leiden University Medical Center
Selma Bozorgpana: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Marcel Tijsterman: Department of Human Genetics, Leiden University Medical Center
Lars Feuk: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Ulf Gyllensten: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Marcel den Hoed: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University
Adam Ameur: Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University

DOI: https://doi.org/10.1038/s41467-022-28244-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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CRISPR-Cas9 can introduce unintended off-target effects. Here authors show that unintended mutations produced by in vivo of zebrafish can be inherited by their off-spring.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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