STAR Protocols (Sep 2024)

Protocol for genetic engineering in Drosophila suzukii using microinjection

  • Ying Yan,
  • Marc F. Schetelig

Journal volume & issue
Vol. 5, no. 3
p. 103248

Abstract

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Summary: The spotted wing Drosophila (Drosophila suzukii Matsumura) is recognized globally as a significant economic pest. Here, we present a protocol for genetic engineering in D. suzukii using microinjection. We describe steps for genetic engineering techniques, including transposon-mediated germline transformation, recombinase-mediated genome targeting, and CRISPR-mediated gene editing. This protocol can significantly expand the toolkit for functional genomics and genetic control studies of this pest.For complete details on the use and execution of this protocol, please refer to Schetelig and Handler,1 Schetelig et al.2 Yan et al.,3 and Yan et al.4 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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