Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)

Benjamin Carter; Wai Lim Ku; Jee Youn Kang; Gangqing Hu; Jonathan Perrie; Qingsong Tang; Keji Zhao

doi:10.1038/s41467-019-11559-1

Nature Communications (Aug 2019)

Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)

Benjamin Carter,
Wai Lim Ku,
Jee Youn Kang,
Gangqing Hu,
Jonathan Perrie,
Qingsong Tang,
Keji Zhao

Affiliations

Benjamin Carter: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Wai Lim Ku: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Jee Youn Kang: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Gangqing Hu: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Jonathan Perrie: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Qingsong Tang: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH
Keji Zhao: Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH

DOI: https://doi.org/10.1038/s41467-019-11559-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 5

Abstract

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The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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