Mitochondrial DNA. Part B. Resources (Oct 2024)

The complete mitogenome of Alternaria tenuissima (Kunze) Wiltshire 1933 (Pleosporaceae), a fungus causing apple leaf blotch disease

  • Chen Tan,
  • Yan Feng,
  • Jing Peng,
  • Jianmei Li,
  • Xiangdong Zhang,
  • Aihua Fu,
  • Wei Tang

DOI
https://doi.org/10.1080/23802359.2024.2419449
Journal volume & issue
Vol. 9, no. 10
pp. 1445 – 1449

Abstract

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Alternaria tenuissima (Kunze) Wiltshire 1933 is a plant pathogenic fungus mainly causing leaf blotch disease. Here, we de novo assembled mitochondrial genome of A. tenuissima isolate AT-1224. The total mitogenome size is 57,475 bp with 29.00% G + C content. The genome contained 12 coding genes and 15 hypothetical proteins, 34 transfer RNA (tRNA) genes and 2 ribosomal RNA (rRNA). There are 227 SSR repeats, range from 2 to 4 base pairs, most five repeats were AT (144), AAT (54), AG (33), AC (13) and AAG (5). The results also found 13 tandem repeats (>100 bp), the largest repeat were forward 2 times located from 13,405 to 20,024 bp and 25,549 to 32,168 bp. Phylogenetic analysis based on 17 species complete mitogenomes indicated that A. tenuissima mitogenome was closest to 2 species, A. solani and A. alternata, sister clade to 6 species, representing Curvularia clavate, Exserohilum rostratum, Exserohilum turcicum, Bipolaris cookie, Bipolaris oryzae and Bipolaris sorokiniana. Further analysis among common fungus in local apple orchards using mitochondrial protein-coding genes revealed A. tenuissima were closing to 2 Alternaria fungi and a fungus representing Phoma sp. These results provide a basic reference for identification and evolution studies of A. tenuissima on apple trees.

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