Nature Communications (Aug 2017)

Single molecule high-throughput footprinting of small and large DNA ligands

  • Maria Manosas,
  • Joan Camunas-Soler,
  • Vincent Croquette,
  • Felix Ritort

DOI
https://doi.org/10.1038/s41467-017-00379-w
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 12

Abstract

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Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small drugs and large proteins.