Nature Communications (May 2020)
Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa
- Haitao Chen,
- Yan Zeng,
- Yongzhi Yang,
- Lingli Huang,
- Bolin Tang,
- He Zhang,
- Fei Hao,
- Wei Liu,
- Youhan Li,
- Yanbin Liu,
- Xiaoshuang Zhang,
- Ru Zhang,
- Yesheng Zhang,
- Yongxin Li,
- Kun Wang,
- Hua He,
- Zhongkai Wang,
- Guangyi Fan,
- Hui Yang,
- Aike Bao,
- Zhanhuan Shang,
- Jianghua Chen,
- Wen Wang,
- Qiang Qiu
Affiliations
- Haitao Chen
- State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences
- Yan Zeng
- State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences
- Yongzhi Yang
- State Key Laboratory of Grassland Agro-Ecosystem, Lanzhou University
- Lingli Huang
- Guangdong Sanjie Forage Biotechnology Co., Ltd.
- Bolin Tang
- Guangdong Sanjie Forage Biotechnology Co., Ltd.
- He Zhang
- BGI-Qingdao
- Fei Hao
- Center of Special Environmental Biomechanics & Biomedical Engineering, School of Life Sciences, Northwestern Polytechnical University
- Wei Liu
- State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences
- Youhan Li
- CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence in Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden
- Yanbin Liu
- State Key Laboratory of Grassland Agro-Ecosystem, Lanzhou University
- Xiaoshuang Zhang
- Guangdong Sanjie Forage Biotechnology Co., Ltd.
- Ru Zhang
- School of Ecology and Environment, Northwestern Polytechnical University
- Yesheng Zhang
- State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences
- Yongxin Li
- School of Ecology and Environment, Northwestern Polytechnical University
- Kun Wang
- School of Ecology and Environment, Northwestern Polytechnical University
- Hua He
- CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence in Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden
- Zhongkai Wang
- School of Ecology and Environment, Northwestern Polytechnical University
- Guangyi Fan
- BGI-Qingdao
- Hui Yang
- Center of Special Environmental Biomechanics & Biomedical Engineering, School of Life Sciences, Northwestern Polytechnical University
- Aike Bao
- State Key Laboratory of Grassland Agro-Ecosystem, Lanzhou University
- Zhanhuan Shang
- State Key Laboratory of Grassland Agro-Ecosystem, Lanzhou University
- Jianghua Chen
- CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, CAS Center for Excellence in Molecular Plant Sciences, Xishuangbanna Tropical Botanical Garden
- Wen Wang
- State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences
- Qiang Qiu
- School of Ecology and Environment, Northwestern Polytechnical University
- DOI
- https://doi.org/10.1038/s41467-020-16338-x
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Alfalfa is an important forage crop, but genetic improvement is challenging due to the lack of a reference genome and an efficient genome editing protocol. Here, the authors report the chromosome-level assembly of the autotetraploid genome and a CRISPR/Cas9-based transgene-free genome editing protocol.
