Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Yicong Wu; Abhishek Kumar; Corey Smith; Evan Ardiel; Panagiotis Chandris; Ryan Christensen; Ivan Rey-Suarez; Min Guo; Harshad D. Vishwasrao; Jiji Chen; Jianyong Tang; Arpita Upadhyaya; Patrick J. La Riviere; Hari Shroff

doi:10.1038/s41467-017-01250-8

Nature Communications (Nov 2017)

Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy

Yicong Wu,
Abhishek Kumar,
Corey Smith,
Evan Ardiel,
Panagiotis Chandris,
Ryan Christensen,
Ivan Rey-Suarez,
Min Guo,
Harshad D. Vishwasrao,
Jiji Chen,
Jianyong Tang,
Arpita Upadhyaya,
Patrick J. La Riviere,
Hari Shroff

Affiliations

Yicong Wu: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Abhishek Kumar: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Corey Smith: Department of Radiology, University of Chicago
Evan Ardiel: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Panagiotis Chandris: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Ryan Christensen: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Ivan Rey-Suarez: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Min Guo: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Harshad D. Vishwasrao: Advanced Imaging and Microscopy Resource, National Institutes of Health
Jiji Chen: Advanced Imaging and Microscopy Resource, National Institutes of Health
Jianyong Tang: JT Scientific Consulting LLC
Arpita Upadhyaya: Biophysics Program, University of Maryland
Patrick J. La Riviere: Department of Radiology, University of Chicago
Hari Shroff: Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-017-01250-8
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

Read online

Light-sheet fluorescence microscopy enables high resolution imaging of biological samples. Here the authors use reflective coverslips to obtain multiple sample views simultaneously, improving the speed of acquisition and resolution compared to dual-view selective plane illumination microscopy.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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