Biomedicines (Apr 2021)

New Insights of the NEET Protein CISD2 Reveals Distinct Features Compared to Its Close Mitochondrial Homolog mitoNEET

  • Myriam Salameh,
  • Sylvie Riquier,
  • Olivier Guittet,
  • Meng-Er Huang,
  • Laurence Vernis,
  • Michel Lepoivre,
  • Marie-Pierre Golinelli-Cohen

DOI
https://doi.org/10.3390/biomedicines9040384
Journal volume & issue
Vol. 9, no. 4
p. 384

Abstract

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Human CISD2 and mitoNEET are two NEET proteins anchored in the endoplasmic reticulum and mitochondria membranes respectively, with an Fe–S containing domain stretching out in the cytosol. Their cytosolic domains are close in sequence and structure. In the present study, combining cellular and biochemical approaches, we compared both proteins in order to possibly identify specific roles and mechanisms of action in the cell. We show that both proteins exhibit a high intrinsic stability and a sensitivity of their cluster to oxygen. In contrast, they differ in according to expression profiles in tissues and intracellular half-life. The stability of their Fe–S cluster and its ability to be transferred in vitro are affected differently by pH variations in a physiological and pathological range for cytosolic pH. Finally, we question a possible role for CISD2 in cellular Fe–S cluster trafficking. In conclusion, our work highlights unexpected major differences in the cellular and biochemical features between these two structurally close NEET proteins.

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