Moringa oleifera decreases biomarkers of oxidative stress in a murine NASH model

Abstract

Introduction and Objectives: This study aimed to evaluate the effect of Moringa oleifera aqueous extract on biomarkers of oxidative stress in a murine model of NASH. Material and Methods: The characterization of the extract was performed by DPPH and ABTS spectrophotometric assays. Male C57BL/6J mice were randomly separated into two groups. 1) Standard diet (ND) (n = 5) (18% lipid) and 2) High fat (HF) diet (n = 10) (60% lipid and 42 g/L sugar in water of use), for 16 weeks. At the end of eight weeks, five HF group mice were divided into a subgroup, 3) Moringa Oleifera (HF + MO), 290 mg/kg/day p.o. for eight weeks. Malondialdehyde (MDA) levels were determined in liver homogenates and the transcriptome by microarray. Differences between groups were determined by ANOVA/Kruskal-Wallis test. The trial was approved by the research ethics committee. Results: Moringa aqueous extract showed antioxidant capacity; DPPH values were 10081.4 0.3 and 22960.4 0.3 for ABTS. Hepatic MDA levels were increased in the HF group compared to the ND group (p<0.05) and decreased in the moringa-treated group (p<0.05). In the transcriptome, mRNAs involved in endoplasmic reticulum stress were underexpressed. Discussion: An increase in MDA has been demonstrated in a murine model of NAFLD induced by a high-fat diet. In our study, Moringa administration reduces MDA production and gene expression of molecules involved in oxidative stress. Conclusions: MO treatment is a therapeutic alternative for the NASH spectrum of liver disorders. Funding: The resources used in this study were from the hospital without any additional financing Declaration of interest: The authors declare no potential conflicts of interest.