Association between plasma phospholipid saturated fatty acids and metabolic markers of lipid, hepatic, inflammation and glycaemic pathways in eight European countries: a cross-sectional analysis in the EPIC-InterAct study
Ju-Sheng Zheng,
Stephen J. Sharp,
Fumiaki Imamura,
Albert Koulman,
Matthias B. Schulze,
Zheng Ye,
Jules Griffin,
Marcela Guevara,
José María Huerta,
Janine Kröger,
Ivonne Sluijs,
Antonio Agudo,
Aurelio Barricarte,
Heiner Boeing,
Sandra Colorado-Yohar,
Courtney Dow,
Miren Dorronsoro,
Pia T. Dinesen,
Guy Fagherazzi,
Paul W. Franks,
Edith J. M. Feskens,
Tilman Kühn,
Verena Andrea Katzke,
Timothy J. Key,
Kay-Tee Khaw,
Maria Santucci de Magistris,
Francesca Romana Mancini,
Elena Molina-Portillo,
Peter M. Nilsson,
Anja Olsen,
Kim Overvad,
Domenico Palli,
Jose Ramón Quirós,
Olov Rolandsson,
Fulvio Ricceri,
Annemieke M. W. Spijkerman,
Nadia Slimani,
Giovanna Tagliabue,
Anne Tjonneland,
Rosario Tumino,
Yvonne T. van der Schouw,
Claudia Langenberg,
Elio Riboli,
Nita G. Forouhi,
Nicholas J. Wareham
Affiliations
Ju-Sheng Zheng
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Stephen J. Sharp
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Fumiaki Imamura
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Albert Koulman
MRC Elsie Widdowson Laboratory
Matthias B. Schulze
Department of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke
Zheng Ye
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Jules Griffin
Department of Biochemistry, University of Cambridge
Marcela Guevara
Navarra Public Health Institute (ISPN)
José María Huerta
CIBER Epidemiology and Public Health (CIBERESP)
Janine Kröger
Department of Molecular Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke
Ivonne Sluijs
University Medical Center Utrecht
Antonio Agudo
Unit of Nutrition and Cancer, Cancer Epidemiology Research Program, Catalan Institute of Oncology-IDIBELL, L’Hospitalet de Llobregat
Aurelio Barricarte
Navarra Public Health Institute (ISPN)
Heiner Boeing
Department of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke
Sandra Colorado-Yohar
Navarra Institute for Health Research (ldiSNA)
Courtney Dow
INSERM U1018, Center for Research in Epidemiology and Population Health
Miren Dorronsoro
Public Health Division of Gipuzkoa
Pia T. Dinesen
Department of Cardiology, Aalborg University Hospital
Guy Fagherazzi
INSERM U1018, Center for Research in Epidemiology and Population Health
Paul W. Franks
Lund University
Edith J. M. Feskens
Wageningen University
Tilman Kühn
German Cancer Research Center (DKFZ), Division of Cancer Epidemiology
Verena Andrea Katzke
German Cancer Research Center (DKFZ), Division of Cancer Epidemiology
Timothy J. Key
Cancer Epidemiology Unit, Nuffield Department of Population Health, University of Oxford
Kay-Tee Khaw
Department of Public Health and Primary Care, University of Cambridge
Maria Santucci de Magistris
A.O.U. Federico II
Francesca Romana Mancini
INSERM U1018, Center for Research in Epidemiology and Population Health
Elena Molina-Portillo
CIBER Epidemiology and Public Health (CIBERESP)
Peter M. Nilsson
Lund University
Anja Olsen
Danish Cancer Society Research Center
Kim Overvad
Department of Cardiology, Aalborg University Hospital
Domenico Palli
Cancer Research and Prevention Institute (ISPO)
Jose Ramón Quirós
Public Health Directorate
Olov Rolandsson
Umeå University
Fulvio Ricceri
Department of Clinical and Biological Sciences, University of Turin
Annemieke M. W. Spijkerman
National Institute for Public Health and the Environment (RIVM)
Nadia Slimani
International Agency for Research on Cancer
Giovanna Tagliabue
Fondazione IRCCS Istituto Nazionale dei Tumori
Anne Tjonneland
Danish Cancer Society Research Center
Rosario Tumino
Cancer Registry and Histopathology Department, “Civic M.P. Arezzo” Hospital, ASP
Yvonne T. van der Schouw
University Medical Center Utrecht
Claudia Langenberg
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Elio Riboli
School of Public Health, Imperial College London
Nita G. Forouhi
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Nicholas J. Wareham
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine
Abstract Background Accumulating evidence suggests that individual circulating saturated fatty acids (SFAs) are heterogeneous in their associations with cardio-metabolic diseases, but evidence about associations of SFAs with metabolic markers of different pathogenic pathways is limited. We aimed to examine the associations between plasma phospholipid SFAs and the metabolic markers of lipid, hepatic, glycaemic and inflammation pathways. Methods We measured nine individual plasma phospholipid SFAs and derived three SFA groups (odd-chain: C15:0 + C17:0, even-chain: C14:0 + C16:0 + C18:0, and very-long-chain: C20:0 + C22:0 + C23:0 + C24:0) in individuals from the subcohort of the European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct case-cohort study across eight European countries. Using linear regression in 15,919 subcohort members, adjusted for potential confounders and corrected for multiple testing, we examined cross-sectional associations of SFAs with 13 metabolic markers. Multiplicative interactions of the three SFA groups with pre-specified factors, including body mass index (BMI) and alcohol consumption, were tested. Results Higher levels of odd-chain SFA group were associated with lower levels of major lipids (total cholesterol (TC), triglycerides, apolipoprotein A-1 (ApoA1), apolipoprotein B (ApoB)) and hepatic markers (alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT)). Higher even-chain SFA group levels were associated with higher levels of low-density lipoprotein cholesterol (LDL-C), TC/high-density lipoprotein cholesterol (HDL-C) ratio, triglycerides, ApoB, ApoB/A1 ratio, ALT, AST, GGT and CRP, and lower levels of HDL-C and ApoA1. Very-long-chain SFA group levels showed inverse associations with triglycerides, ApoA1 and GGT, and positive associations with TC, LDL-C, TC/HDL-C, ApoB and ApoB/A1. Associations were generally stronger at higher levels of BMI or alcohol consumption. Conclusions Subtypes of SFAs are associated in a differential way with metabolic markers of lipid metabolism, liver function and chronic inflammation, suggesting that odd-chain SFAs are associated with lower metabolic risk and even-chain SFAs with adverse metabolic risk, whereas mixed findings were obtained for very-long-chain SFAs. The clinical and biochemical implications of these findings may vary by adiposity and alcohol intake.