Protocol to study presynaptic protein synthesis in ex vivo mouse hippocampal slices using HaloTag technology

Abstract

Summary: Presynaptic boutons in the mammalian brain are typically small and difficult to manipulate and study. Here, we present a protocol applying HaloTag self-labeling technology to detect de novo local protein synthesis in intact presynaptic mossy fiber boutons from acute mouse hippocampal slices. We describe stereotaxic injection of HaloTag-expressing virus into the brain region of interest, followed by brain slice preparation. We then detail the labeling of HaloTag-fused protein and image acquisition to visualize the labeled protein in an intact circuit.For complete details on the use and execution of this protocol, please refer to Monday et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Microscopy
• Molecular Biology
• Neuroscience
• Molecular/Chemical Probes