Scaffold-free human mesenchymal stem cell construct geometry regulates long bone regeneration

Samuel Herberg; Daniel Varghai; Daniel S. Alt; Phuong N. Dang; Honghyun Park; Yuxuan Cheng; Jung-Youn Shin; Anna D. Dikina; Joel D. Boerckel; Marsha W. Rolle; Eben Alsberg

doi:10.1038/s42003-020-01576-y

Communications Biology (Jan 2021)

Scaffold-free human mesenchymal stem cell construct geometry regulates long bone regeneration

Samuel Herberg,
Daniel Varghai,
Daniel S. Alt,
Phuong N. Dang,
Honghyun Park,
Yuxuan Cheng,
Jung-Youn Shin,
Anna D. Dikina,
Joel D. Boerckel,
Marsha W. Rolle,
Eben Alsberg

Affiliations

Samuel Herberg: Department of Biomedical Engineering, Case Western Reserve University
Daniel Varghai: Department of Biomedical Engineering, Case Western Reserve University
Daniel S. Alt: Department of Biomedical Engineering, Case Western Reserve University
Phuong N. Dang: Department of Biomedical Engineering, Case Western Reserve University
Honghyun Park: Department of Biomedical Engineering, Case Western Reserve University
Yuxuan Cheng: Department of Biomedical Engineering, Case Western Reserve University
Jung-Youn Shin: Department of Biomedical Engineering, Case Western Reserve University
Anna D. Dikina: Department of Biomedical Engineering, Case Western Reserve University
Joel D. Boerckel: Department of Aerospace and Mechanical Engineering, University of Notre Dame
Marsha W. Rolle: Department of Biomedical Engineering, Worcester Polytechnic Institute
Eben Alsberg: Department of Biomedical Engineering, Case Western Reserve University

DOI: https://doi.org/10.1038/s42003-020-01576-y
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 16

Abstract

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Herberg et al. previously showed functional healing of femoral defects using scaffold-free human mesenchymal stem cell (hMSC) condensates with localized morphogen presentation. In this study, they report the importance of the tubular geometry of MSC condensates in long bone regeneration. Unlike loosely packed hMSC sheets, only hMSC tubes induced regenerate tissue partially resembling normal growth plate.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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