Study the effects of pectin, medium type and plant growth regulators (PGRs) on micropropagation of GF677 under in vitro condition

Abstract

GF677 hybrid is one the suitable somaclone rootstock for almond and peach in worldwide, and its mass-propagation is demanded highly. For its in vitro micro-propagation MS medium is used, in which it critically involves with the some problems such as low shooting efficiency, vitrification and rosetting growth. In order to overcome to these problems the effects of three concentrations of pectin, three types of media and three plant growth regulators (PGRs) at different concentration on the rate of adventitious shooting and quality using the axial buds and factorial experimental design under in vitro condition was investigated. The results indicated that the rate of adventitious shoot regeneration on TK and WPM media contain pectin is high and on MS contain pectin is low; but on contrary, the rate and quality of normal growth of shootlets was vice versa. The combination of 0.5mg/l ZR and 0.25 mg/l BAP on MS contain 0.5% pectin exhibited as the best treatment for the shooting rate and normal growth of shootlets, in which it seems that the BAP mostly responsible for shoot regeneration and normal leaves development and the ZR responsible for inter node growth and increasing shoot length. However, IBA has no significance effect on mentioned characters in comparison with other PGRs in this context.

Keywords

• gf677
• pectin
• plant growth regulators (pgrs)
• micropropagation