Protocol for inducing epidermis-specific gene deletion and establishing a UVB-irradiated skin inflammation model

Abstract

Summary: When skin is exposed to ultraviolet B (UVB) radiation, damaged keratinocytes release double-stranded RNA to trigger an inflammatory response. Here, we present a protocol to induce epidermal-specific Zfp750 gene-deficient mice and establish a UVB-induced skin inflammation model. We detail procedures for detecting gene expressions in the epidermis by quantitative reverse-transcription PCR (RT-qPCR) and analyzing changes in skin immune cell subsets after UVB radiation using flow cytometry.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Genetics
• Immunology
• Model Organisms