Инфекция и иммунитет (Jul 2014)

ENTEROBACTERIACAE, PRODUCING ESBLS AND METALLO-β-LACTAMASE NDM-1, ISOLATED IN HOSPITALS OF BALTIC REGION COUNTRIES

  • S. A. Egorova,
  • L. A. Kaftyreva,
  • L. V. Lipskaya,
  • I. B. Konovalenko,
  • M. F. Pyasetskaya,
  • T. S. Kurchikova,
  • N. B. Vedernikova,
  • O. T. Morozova,
  • M. V. Smirnova,
  • L. N. Popenko,
  • M. I. Lubushkina,
  • J. A. Savochkina,
  • M. A. Makarova,
  • L. V. Suzhaeva,
  • J. V. Ostankova,
  • M. N. Ivanova,
  • A. M. Pavelkovich,
  • P. Naaber,
  • E. Sepp,
  • S. Kõljalg,
  • J. Miciuleviciene,
  • A. Balode

DOI
https://doi.org/10.15789/2220-7619-2013-1-29-36
Journal volume & issue
Vol. 3, no. 1
pp. 29 – 36

Abstract

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Abstract. We studied the prevalence of K. pneumoniae and E. coli resistance to extended spectrum cephalosporins and carbapenems, isolated from patients of eight hospitals in St-Petersburg from January to May, 2012. Prevalence of cephalosporin resistant isolates varied in different hospitals: E. coli – from 7,8 to 50%, K. pneumoniae – from 25,4 to 88,4%. Isolates produced extended spectrum beta-lactamases СТХ-М, mainly СТХ-М-1, also СТХ-М-2 and СТХ-М-9. Twenty two carbapenem-resistant K. pneumoniae strains (also resistant to other antimicrobials) were isolated in three hospitals. MALDI-TOF MS showed that carbapenem resistance was caused by carbapenemase. Carbapenemases of all isolates belonged to metallo-β-lactamases according to results of the ROSCO Diagnostica tests. The gene coding production of New Delhi metallo-β-lactamase (blaNDM-1) were detected in all strains. Our data confirmed that the main cephalosporin resistance mechanism of E. coli и K. pneumoniae in Baltic region (including Russia, St-Petersburg) is CTX-M-1 production. For the first time in Russia K. pneumoniae strains producing metallo-β-lactamases NDM-1 were isolated in several hospitals of St-Petersburg.

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