Nature Communications (Mar 2023)
Improving adenine and dual base editors through introduction of TadA-8e and Rad51DBD
- Niannian Xue,
- Xu Liu,
- Dan Zhang,
- Youming Wu,
- Yi Zhong,
- Jinxin Wang,
- Wenjing Fan,
- Haixia Jiang,
- Biyun Zhu,
- Xiyu Ge,
- Rachel V. L. Gonzalez,
- Liang Chen,
- Shun Zhang,
- Peilu She,
- Zhilin Zhong,
- Jianjian Sun,
- Xi Chen,
- Liren Wang,
- Zhimin Gu,
- Ping Zhu,
- Mingyao Liu,
- Dali Li,
- Tao P. Zhong,
- Xiaohui Zhang
Affiliations
- Niannian Xue
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Xu Liu
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Dan Zhang
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Youming Wu
- Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
- Yi Zhong
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Jinxin Wang
- Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
- Wenjing Fan
- Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
- Haixia Jiang
- School of Life Sciences & Biotechnology, Shanghai Jiao Tong University
- Biyun Zhu
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Xiyu Ge
- Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign
- Rachel V. L. Gonzalez
- Department of Physiology and Cellular Biophysics, Columbia University
- Liang Chen
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Shun Zhang
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Peilu She
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Zhilin Zhong
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Jianjian Sun
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Xi Chen
- BRL Medicine, Inc.
- Liren Wang
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Zhimin Gu
- Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
- Ping Zhu
- Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences
- Mingyao Liu
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Dali Li
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Tao P. Zhong
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- Xiaohui Zhang
- Shanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University
- DOI
- https://doi.org/10.1038/s41467-023-36887-1
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 12
Abstract
There is a low efficiency of A-to-G base conversion in at specific positions using base editors. Here the authors fuse ABE8e with the Rad51 DNA-binding domain to generate a hyperactive ABE allowing improved A-to-G editing efficiency at the region proximal to the PAM and improved simultaneous A/C conversion efficiency.
