Armaghane Danesh Bimonthly Journal (Mar 2022)
Investigating Anticancer Effects of Silver Nanoparticles on Bladder Cancer 5637 Cells in Comparison to Human Embryonic Kidney Normal Cells (HEK-293)
Abstract
Background & aim: Nanotechnology is a new research field with wide applications in cancer management. Among the various metal nanoparticles, silver nanoparticles have been used to treat many cancers due to their high antitumor potential. Despite the potential benefits of these nanoparticles, the extent to which they affect normal cells has become a challenge. In addition, their anti-cancer effects on 5637 bladder cancer cells have not been well established. The aim of the present study was to determine the anti-cancer effects of silver nanoparticles on the survival of 5637 bladder cancer tumor cells in comparison with normal embryonic kidney cells HEK-293. Methods: In the present experimental study performed in 2021, the survival of 5637 cells of bladder cancer and normal cells of embryonic kidney (HEK-293) 24 hours after treatment with 30-50 nm silver nanoparticles with concentrations (0-125). Micrograms per milliliter was evaluated by MTT(dimethyl thiazole-diphenyltetrazolium bromide) test. Morphological changes were also assessed by light microscopy. VEGFA gene expression level and cell migration rate were evaluated by quantitative polymerase chain reaction and scratch testing, respectively. The collected data were analyzed using Shapiro-Wilk statistical tests, one-way and two-way analysis of variance, Tukey post hoc test. Results: The results of the present study indicated that the reduction in survival in 5637 and HEK-293 cells after treatment with silver nanoparticles was dose-dependent, which significantly decreased in 5637 tumor cells. HEK-293 was more than normal cells (p <0.05). In addition, treatment with concentrations of 50 and 60 μg / ml silver nanoparticles significantly reduced VEGFA gene expression (p<0.05) and inhibited the migration of 5637 bladder cancer cells (p<0.001). Conclusion: AgNPs could reduce the viability of 5637 and HEK-293 cells as their inhibitory effects on 5637 cells viability were significantly more than HEK-293. Furthermore, AgNPs suppressed the 5637 cells migration.
