Efficiency of Vanilla, Patchouli and Ylang Ylang Essential Oils Stabilized by Iron Oxide@C14 Nanostructures against Bacterial Adherence and Biofilms Formed by Staphylococcus aureus and Klebsiella pneumoniae Clinical Strains
Maxim Bilcu,
Alexandru Mihai Grumezescu,
Alexandra Elena Oprea,
Roxana Cristina Popescu,
George Dan Mogoșanu,
Radu Hristu,
George A. Stanciu,
Dan Florin Mihailescu,
Veronica Lazar,
Eugenia Bezirtzoglou,
Mariana Carmen Chifiriuc
Affiliations
Maxim Bilcu
Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
Alexandra Elena Oprea
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
Roxana Cristina Popescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
George Dan Mogoșanu
Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania
Radu Hristu
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
George A. Stanciu
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Dan Florin Mihailescu
Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Veronica Lazar
Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Eugenia Bezirtzoglou
Laboratory of Microbiology, Biotechnology and Hygiene, Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece
Mariana Carmen Chifiriuc
Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Biofilms formed by bacterial cells are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence and chronicization of the microbial infections and to therapy failure. The purpose of this study was to combine the unique properties of magnetic nanoparticles with the antimicrobial activity of three essential oils to obtain novel nanobiosystems that could be used as coatings for catheter pieces with an improved resistance to Staphylococcus aureus and Klebsiella pneumoniae clinical strains adherence and biofilm development. The essential oils of ylang ylang, patchouli and vanilla were stabilized by the interaction with iron oxide@C14 nanoparticles to be further used as coating agents for medical surfaces. Iron oxide@C14 was prepared by co-precipitation of Fe+2 and Fe+3 and myristic acid (C14) in basic medium. Vanilla essential oil loaded nanoparticles pelliculised on the catheter samples surface strongly inhibited both the initial adherence of S. aureus cells (quantified at 24 h) and the development of the mature biofilm quantified at 48 h. Patchouli and ylang-ylang essential oils inhibited mostly the initial adherence phase of S. aureus biofilm development. In the case of K. pneumoniae, all tested nanosystems exhibited similar efficiency, being active mostly against the adherence K. pneumoniae cells to the tested catheter specimens. The new nanobiosystems based on vanilla, patchouli and ylang-ylang essential oils could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with anti-adherence and anti-biofilm properties.
