Toxoplasma membrane inositol phospholipid binding protein TgREMIND is essential for secretory organelle function and host infection
Rodrigue Houngue,
Lamba Omar Sangaré,
Tchilabalo Dilezitoko Alayi,
Aissatou Dieng,
Tristan Bitard-Feildel,
Claire Boulogne,
Christian Slomianny,
Cynthia Menonve Atindehou,
Lucie Ayi Fanou,
Yetrib Hathout,
Isabelle Callebaut,
Stanislas Tomavo
Affiliations
Rodrigue Houngue
Université Paris Saclay, CNRS UMR 9198-CEA, Institute for Integrative Biology of the Cell (I2BC), 91190 Gif sur Yvette, France
Lamba Omar Sangaré
Department of Biology, Texas A&M University, College Station, TX 77843, USA
Tchilabalo Dilezitoko Alayi
Department of Pharmaceutical Science, School of Pharmacy and Pharmaceutical Sciences, Binghamton University−SUNY, Johnson City, NY 13790, USA
Aissatou Dieng
Université Paris Saclay, CNRS UMR 9198-CEA, Institute for Integrative Biology of the Cell (I2BC), 91190 Gif sur Yvette, France
Tristan Bitard-Feildel
Sorbonne Université, Muséum National d'Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC, 75005 Paris, France
Claire Boulogne
Université Paris Saclay, CNRS UMR 9198-CEA, Institute for Integrative Biology of the Cell (I2BC), 91190 Gif sur Yvette, France; Plateforme Imagerie-Gif, Institut de Biologie Intégrative de la Cellule (I2BC), 91190 Gif sur Yvette, France
Christian Slomianny
University of Lille, Laboratory of Cell Physiology, INSERM U 1003, 59655 Villeneuve d'Ascq, France
Cynthia Menonve Atindehou
Université d’Abomey Calavi, Laboratoire de Biochimie et de Biologie Moléculaire, Faculté des Sciences et Technologies, Cotonou, Bénin
Lucie Ayi Fanou
Université d’Abomey Calavi, Laboratoire de Biochimie et de Biologie Moléculaire, Faculté des Sciences et Technologies, Cotonou, Bénin
Yetrib Hathout
Department of Pharmaceutical Science, School of Pharmacy and Pharmaceutical Sciences, Binghamton University−SUNY, Johnson City, NY 13790, USA
Isabelle Callebaut
Sorbonne Université, Muséum National d'Histoire Naturelle, UMR CNRS 7590, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, IMPMC, 75005 Paris, France
Stanislas Tomavo
Université Paris Saclay, CNRS UMR 9198-CEA, Institute for Integrative Biology of the Cell (I2BC), 91190 Gif sur Yvette, France; Corresponding author
Summary: Apicomplexan parasites possess specialized secretory organelles called rhoptries, micronemes, and dense granules that play a vital role in host infection. In this study, we demonstrate that TgREMIND, a protein found in Toxoplasma gondii, is necessary for the biogenesis of rhoptries and dense granules. TgREMIND contains a Fes-CIP4 homology-Bin/Amphiphysin/Rvs (F-BAR) domain, which binds to membrane phospholipids, as well as a novel uncharacterized domain that we have named REMIND (regulator of membrane-interacting domain). Both the F-BAR domain and the REMIND are crucial for TgREMIND functions. When TgREMIND is depleted, there is a significant decrease in the abundance of dense granules and abnormal transparency of rhoptries, leading to a reduction in protein secretion from these organelles. The absence of TgREMIND inhibits host invasion and parasite dissemination, demonstrating that TgREMIND is essential for the proper function of critical secretory organelles required for successful infection by Toxoplasma.
