Journal of Animal Reproduction and Biotechnology (Mar 2014)

In Vitro Development of Porcine Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa with Trehalose

  • Hwa-Hyung Kang,
  • Ji-Woong Lee,
  • Man-Jong Kang,
  • Kwang-Hyun Kim,
  • Seung-Ju Moon

DOI
https://doi.org/10.12750/jet.2014.29.1.51
Journal volume & issue
Vol. 29, no. 1
pp. 51 – 57

Abstract

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The objectives of this study were to investigate the effects of trehalose as a cryoprotectant for porcine freeze-dried spermatozoa, to find the optimal freeze-drying time and storage periods of freeze-dried spermatozoa, and to find out pronuclear formation rates, cleaved rates, and embryo development through intracytoplasmic injection of freeze-dried spermatozoa on porcine oocytes. The survival rates of spermatozoa after freeze-drying with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). The highest survival rates were found at 75 mM trehalose treatment. The longer storage periods after freeze-drying seemed to have a lower survival rates. Development in culture of pig by ICSI with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). Shorter freeze-drying time of spermatozoa was resulted in the highest cleaved rates and embryo development.

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