A VSV-based assay quantifies coronavirus Mpro/3CLpro/Nsp5 main protease activity and chemical inhibition

Emmanuel Heilmann; Francesco Costacurta; Stephan Geley; Seyad Arad Mogadashi; Andre Volland; Bernhard Rupp; Reuben Stewart Harris; Dorothee von Laer

doi:10.1038/s42003-022-03277-0

Communications Biology (Apr 2022)

A VSV-based assay quantifies coronavirus Mpro/3CLpro/Nsp5 main protease activity and chemical inhibition

Emmanuel Heilmann,
Francesco Costacurta,
Stephan Geley,
Seyad Arad Mogadashi,
Andre Volland,
Bernhard Rupp,
Reuben Stewart Harris,
Dorothee von Laer

Affiliations

Emmanuel Heilmann: Institute of Virology, Medical University of Innsbruck
Francesco Costacurta: Institute of Virology, Medical University of Innsbruck
Stephan Geley: Division of Pathophysiology, Medical University of Innsbruck
Seyad Arad Mogadashi: Department of Biochemistry, Molecular Biology and Biophysics, Masonic Cancer Center, Institute for Molecular Virology, Center for Genome Engineering, University of Minnesota
Andre Volland: Institute of Virology, Medical University of Innsbruck
Bernhard Rupp: Institute of Genetic Epidemiology, Medical University of Innsbruck
Reuben Stewart Harris: Department of Biochemistry, Molecular Biology and Biophysics, Masonic Cancer Center, Institute for Molecular Virology, Center for Genome Engineering, University of Minnesota
Dorothee von Laer: Institute of Virology, Medical University of Innsbruck

DOI: https://doi.org/10.1038/s42003-022-03277-0
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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A vesicular stomatitis virus (VSV)-based assay enables high-throughput screening for small molecular protease inhibitors that can block viral proteases, like the Mpro/3CLpro/Nsp5 in SARS-CoV-2.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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