Monosaccharide Constituents of Potato Root Exudate Influence Hatching of the White Potato Cyst Nematode

Abstract

Plants secrete a large array of compounds into the rhizosphere to facilitate interactions with their biotic environment. Some of these exuded compounds stimulate the hatching of obligate plant-parasitic nematodes, ultimately leading to a detrimental effect on the host plant. Determining these cues can help to provide new mechanisms for control and aid nematode management schemes. Here, we show that glucose, fructose, and arabinose, which are all present in potato root exudate (PRE), induce hatching of white potato cyst nematode (Globodera pallida) eggs whereas five other PRE sugars had no effect. Although these monosaccharides resulted in significant hatching, none induced the same level as PRE, suggesting that other components, possibly in combination, contribute to stimulation of nematode hatching. Glucose but not arabinose or fructose was also observed to attract juvenile G. pallida, indicating that these hatch-inducing components can have different roles in different stages of the life cycle. Applying a solution of these monosaccharides to G. pallida-infested soil prepotato planting initiated hatching in the absence of a host. Host absence resulted in nematode mortality and a reduction in the G. pallida population. Therefore, subsequent invasion of the crop postplanting was also reduced compared with untreated soil. Our data suggest that monosaccharide components of PRE play an important role in the hatching and attraction of G. pallida. As a result, the hatch-inducing monosaccharides can be applied as a preplanting treatment to induce hatching and reduce subsequent infection rates.[Figure: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Keywords

• attraction
• hatching
• monosaccharides
• potato cyst nematode
• root exudate