The Deposition of a Lectin from <i>Oreochromis niloticus</i> on the Surface of Titanium Dioxide Nanotubes Improved the Cell Adhesion, Proliferation, and Osteogenic Activity of Osteoblast-like Cells
Keicyanne Fernanda Lessa dos Anjos,
Cynarha Daysy Cardoso da Silva,
Mary Angela Aranda de Souza,
Alessandra Batista de Mattos,
Luana Cassandra Breitenbach Barroso Coelho,
Giovanna Machado,
Janaina Viana de Melo,
Regina Celia Bressan Queiroz de Figueiredo
Affiliations
Keicyanne Fernanda Lessa dos Anjos
Departamento de Microbiologia, Instituto Aggeu Magalhães (FIOCRUZ-PE), Campus da UFPE, Av. Prof. Moraes Rego s/n Cidade Universitária, Recife 50670-420, PE, Brazil
Cynarha Daysy Cardoso da Silva
Departamento de Microbiologia, Instituto Aggeu Magalhães (FIOCRUZ-PE), Campus da UFPE, Av. Prof. Moraes Rego s/n Cidade Universitária, Recife 50670-420, PE, Brazil
Mary Angela Aranda de Souza
Departamento de Microbiologia, Instituto Aggeu Magalhães (FIOCRUZ-PE), Campus da UFPE, Av. Prof. Moraes Rego s/n Cidade Universitária, Recife 50670-420, PE, Brazil
Alessandra Batista de Mattos
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Av. Prof. Luiz Freire, 01. Cidade Universitária, Recife 50740-540, PE, Brazil
Luana Cassandra Breitenbach Barroso Coelho
Centro de Ciências Biológicas, Departamento de Bioquímica, Campus da UFPE, Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego s/n Cidade Universitária, Recife 50670-420, PE, Brazil
Giovanna Machado
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Av. Prof. Luiz Freire, 01. Cidade Universitária, Recife 50740-540, PE, Brazil
Janaina Viana de Melo
Centro de Tecnologias Estratégicas do Nordeste (CETENE), Av. Prof. Luiz Freire, 01. Cidade Universitária, Recife 50740-540, PE, Brazil
Regina Celia Bressan Queiroz de Figueiredo
Departamento de Microbiologia, Instituto Aggeu Magalhães (FIOCRUZ-PE), Campus da UFPE, Av. Prof. Moraes Rego s/n Cidade Universitária, Recife 50670-420, PE, Brazil
Titanium and its alloys are used as biomaterials for medical and dental applications, due to their mechanical and physical properties. Surface modifications of titanium with bioactive molecules can increase the osseointegration by improving the interface between the bone and implant. In this work, titanium dioxide nanotubes (TiO2NTs) were functionalized with a lectin from the plasma of the fish Oreochromis niloticus aiming to favor the adhesion and proliferation of osteoblast-like cells, improving its biocompatibility. The TiO2NTs were obtained by anodization of titanium and annealed at 400 °C for 3 h. The resulting TiO2NTs were characterized by high-resolution scanning electron microscopy. The successful incorporation of OniL on the surface of TiO2NTs, by spin coating, was demonstrated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIE), and attenuated total reflection-Fourier transform infrared spectrum (ATR-FTIR). Our results showed that TiO2NTs were successfully synthesized in a regular and well-distributed way. The modification of TiO2NTs with OniL favored adhesion, proliferation, and the osteogenic activity of osteoblast-like cells, suggesting its use to improve the quality and biocompatibility of titanium-based biomaterials.