基于提高α-亚麻酸得率的高速剪切辅助 水酶法提取紫苏籽油的工艺优化Optimization of extraction process for high α-linolenic acid perilla seed oil using high speed shear assisted aqueous enzymatic method

Abstract

旨在为紫苏籽油的生产提供技术指导，采用高速剪切辅助水酶法提取紫苏籽油。以α-亚麻酸得率为响应值，以固液比、剪切速率、剪切时间、酶处理温度、酶处理时间为自变量，利用单因素试验结合响应面试验优化紫苏籽油提取工艺。结果表明，高速剪切辅助水酶法提取紫苏籽油的最佳工艺条件为固液比1∶ 4、剪切速率12 100 r/min、剪切时间4.2 min、酶处理温度40 ℃、酶处理时间3 h，在最佳工艺条件下α-亚麻酸得率为38.2%。综上，采用高速剪切辅助水酶法可有效提取紫苏籽油，且α-亚麻酸得率较高。 In order to provide technical guidance for the production of perilla seed oil, high speed shear assisted aqueous enzymatic method was used to extract perilla seed oil. With α-linolenic acid yield as response value, solid-liquid ratio, shear rate, shear time, enzyme treatment temperature and enzyme treatment time as independent variables, the extraction process of perilla seed oil was optimized by single factor experiment and response surface experiment. The results showed that the optimum conditions for extracting perilla seed oil by high speed shear assisted aqueous enzymatic method were as follows: solid-liquid ratio 1∶ 4, shear rate 12 100 r/min, shear time 4.2 min, enzyme treatment temperature 40 ℃, and enzyme treatment time 3 h. Under the optimum conditions, the yield of α-linolenic acid was 38.2%. In conclusion, the high speed shear assisted aqueous enzymatic method can effectively extract perilla seed oil, and the yield of α-linolenic acid is higher.

Keywords

• 紫苏籽油；α-亚麻酸；高速剪切；水酶法；响应面试验
• perilla seed oil; α-linolenic acid; high speed shearing; aqueous enzymatic method; response surface experiment