Genome-Wide Identification and Characterization of the Laccase Gene Family in <i>Fragaria vesca</i> and Its Potential Roles in Response to Salt and Drought Stresses
Jingjing Kong,
Rui Xiong,
Keli Qiu,
Xinle Lin,
Debao Li,
Lijuan Lu,
Junyong Zhou,
Shufang Zhu,
Mao Liu,
Qibao Sun
Affiliations
Jingjing Kong
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Rui Xiong
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Keli Qiu
National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China
Xinle Lin
Anhui Province Key Laboratory of Horticultural Crop Quality Biology, Anhui Agricultural University, Hefei 230036, China
Debao Li
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Lijuan Lu
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Junyong Zhou
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Shufang Zhu
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Mao Liu
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Qibao Sun
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230031, China
Laccase (LAC, EC 1.10.3.2) is integral to the formation of lignin synthesis, flavonoid production, and responses to both biotic and abiotic stresses. While recent studies have characterized numerous LAC gene families and their functions across various plants, information regarding LAC genes in woodland strawberry (Fragaria vesca) remains limited. In this study, we identified a total of 57 FvLAC genes in the Fragaria vesca genome, which were phylogenetically categorized into five distinct groups. Analysis of the gene structures revealed a uniformity in the exon–intron structure among the subgroups, while conserved motifs identified unique motifs specific to certain subgroups, suggesting functional variations. Chromosomal localization studies indicated that FvLACs are distributed across seven chromosomes, and collinearity analysis demonstrated that FvLACs exhibit collinearity within the species. Additionally, cis-acting element analysis suggested that FvLAC genes are involved in stress responses, hormone responses, light responses, and the growth and development of plants. qRT-PCR demonstrated that FvLACs responded to salt, drought, and hormone stresses, with the expression levels of FvLAC24, FvLAC32, and FvLAC51 continuously increasing under these stress conditions. Furthermore, transgenic yeast experiments revealed that FvLAC51 enhanced yeast tolerance to both salt and drought stresses, while FvLAC24 and FvLAC32 negatively regulated yeast tolerance under these same conditions. These findings provide a theoretical foundation for further investigation into the functions of FvLAC genes in woodland strawberry.
