Association of the essential oil of Cymbopogon citratus (DC) Stapf with nystatin against oral cavity yeasts

Abstract

Abstract Oral thrush or candidiasis is a fairly common infectious fungal disease that is mainly caused by species of the genus Candida spp. In this work, we evaluated the antifungal activity of the essential oil of Cymbopogon citratus (DC) Stapf (lemongrass) and its combination with nystatin against oral cavity yeasts. The oil was extracted by the steam distillation method, and its constituents were quantified. The yeasts were identified using MALDI-TOF MS. The sensitivity to the essential oil and its association with nystatin was verified by the minimum inhibitory concentration (MIC) and checkerboard methods. In the essential oil samples, there were 84.53% of citral. A total of 64.77% of strains were identified as Candida albicans. On susceptibility tests, 83.55% of the yeast isolates were inhibited in concentrations of ≥16 µg/mL of nystatin. C. citratus’s oil was capable of inhibiting and killing all the isolates tested with concentrations that varied from 0.137 to 2.2 mg/mL. The association of oil and nystatin had an additive effect on more than 78% of the strains. The association of this herbal drug with nystatin potentialized the antifungal effect on yeast samples isolated from the oral cavity of oncologic patients.

Keywords

• infectious diseases
• medicinal plants
• Nystatin
• oral Candidiasis
• pharmacological synergism