Nature Communications (Mar 2020)
c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae
- Kyle A. Floyd,
- Calvin K. Lee,
- Wujing Xian,
- Mahmoud Nametalla,
- Aneesa Valentine,
- Benjamin Crair,
- Shiwei Zhu,
- Hannah Q. Hughes,
- Jennifer L. Chlebek,
- Daniel C. Wu,
- Jin Hwan Park,
- Ali M. Farhat,
- Charles J. Lomba,
- Courtney K. Ellison,
- Yves V. Brun,
- Javier Campos-Gomez,
- Ankur B. Dalia,
- Jun Liu,
- Nicolas Biais,
- Gerard C. L. Wong,
- Fitnat H. Yildiz
Affiliations
- Kyle A. Floyd
- Department of Microbiology and Environmental Toxicology, University of California – Santa Cruz
- Calvin K. Lee
- Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California – Los Angeles
- Wujing Xian
- Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California – Los Angeles
- Mahmoud Nametalla
- Department of Biology, Brooklyn College
- Aneesa Valentine
- Department of Biology, Brooklyn College
- Benjamin Crair
- Department of Microbial Pathogenesis, Yale University
- Shiwei Zhu
- Department of Microbial Pathogenesis, Yale University
- Hannah Q. Hughes
- Department of Biology, Indiana University – Bloomington
- Jennifer L. Chlebek
- Department of Biology, Indiana University – Bloomington
- Daniel C. Wu
- Department of Microbiology and Environmental Toxicology, University of California – Santa Cruz
- Jin Hwan Park
- Department of Microbiology and Environmental Toxicology, University of California – Santa Cruz
- Ali M. Farhat
- Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California – Los Angeles
- Charles J. Lomba
- Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California – Los Angeles
- Courtney K. Ellison
- Department of Biology, Indiana University – Bloomington
- Yves V. Brun
- Department of Microbiology, Infectious Diseases, and Immunology, Faculty of Medicine, University of Montreal
- Javier Campos-Gomez
- Cystic Fibrosis Research Center, University of Alabama at Birmingham
- Ankur B. Dalia
- Department of Biology, Indiana University – Bloomington
- Jun Liu
- Department of Microbial Pathogenesis, Yale University
- Nicolas Biais
- Department of Biology, Brooklyn College
- Gerard C. L. Wong
- Departments of Bioengineering, Chemistry and Biochemistry, California Nano Systems Institute, University of California – Los Angeles
- Fitnat H. Yildiz
- Department of Microbiology and Environmental Toxicology, University of California – Santa Cruz
- DOI
- https://doi.org/10.1038/s41467-020-15331-8
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
Biofilm formation by Vibrio cholerae is regulated by c-di-GMP and requires the type IV MSHA pilus. Here, Floyd et al. show that the MSHA pilus is a dynamic system, and that both extension and retraction are directly controlled by c-di-GMP via regulation of activity of the extension ATPase MshE.
