Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation

Teruyoshi Yamashita; Daisuke Kamiya; Nami Kawaraichi; Harufu Toyokawa; Teppei Akaboshi; Makoto Ikeya; Yayoi Toyooka

Stem Cell Research (Apr 2022)

Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation

Teruyoshi Yamashita,
Daisuke Kamiya,
Nami Kawaraichi,
Harufu Toyokawa,
Teppei Akaboshi,
Makoto Ikeya,
Yayoi Toyooka

Affiliations

Teruyoshi Yamashita: T-CiRA Discovery, Takeda Pharmaceutical Co., Ltd., Fujisawa, Kanagawa, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan
Daisuke Kamiya: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan
Nami Kawaraichi: Orizuru Therapeutics Inc., Fujisawa, Kanagawa, Japan
Harufu Toyokawa: Orizuru Therapeutics Inc., Fujisawa, Kanagawa, Japan
Teppei Akaboshi: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan
Makoto Ikeya: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan
Yayoi Toyooka: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan; Corresponding author at: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 26-1 Muraoka-Higashi 2-chome, Fujisawa, Kanagawa 251-8555, Japan.

Journal volume & issue: Vol. 60
p. 102696

Abstract

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SOX10 (SRY-box transcription factor 10) is not only a definitive molecular marker of neural crest cells (NCCs) but also an essential transcription factor for the differentiation of NCCs in vertebrate embryogenesis. Here, we report the establishment of a human SOX10 knock-in reporter iPSC line (SOX10-tdT) by CRISPR/Cas9-mediated homologous recombination, in which the expression of SOX10 can be monitored as tdTomato fluorescence. This iPSC line can provide a useful tool to model the differentiation process of human NCCs in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal