Gene Expression Analysis Reveals Age and Ethnicity Signatures Between Young and Old Adults in Human PBMC

Yang Hu; Yang Hu; Yudai Xu; Lipeng Mao; Lipeng Mao; Wen Lei; Jian Xiang; Lijuan Gao; Junxing Jiang; Li`an Huang; Oscar Junhong Luo; Oscar Junhong Luo; Jinhai Duan; Guobing Chen

doi:10.3389/fragi.2021.797040

Frontiers in Aging (Feb 2022)

Gene Expression Analysis Reveals Age and Ethnicity Signatures Between Young and Old Adults in Human PBMC

Yang Hu,
Yang Hu,
Yudai Xu,
Lipeng Mao,
Lipeng Mao,
Wen Lei,
Jian Xiang,
Lijuan Gao,
Junxing Jiang,
Li`an Huang,
Oscar Junhong Luo,
Oscar Junhong Luo,
Jinhai Duan,
Guobing Chen

Affiliations

Yang Hu: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Yang Hu: NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute, Guangdong Provincial Fertility Hospital, Guangzhou, China
Yudai Xu: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Lipeng Mao: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Lipeng Mao: Department of Systems Biomedical Sciences, School of Medicine, Jinan University, Guangzhou, China
Wen Lei: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Jian Xiang: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Lijuan Gao: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Junxing Jiang: Nanfang Hospital, Southern Medical University, Guangzhou, China
Li`an Huang: Department of Neurology, the First Affiliated Hospital, Jinan University, Guangzhou, China
Oscar Junhong Luo: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China
Oscar Junhong Luo: Department of Systems Biomedical Sciences, School of Medicine, Jinan University, Guangzhou, China
Jinhai Duan: Eastern Department of Neurology of Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guandong, China
Guobing Chen: Institute of Geriatric Immunology, Department of Microbiology and Immunology, School of Medicine, Jinan University, Guangzhou, China

DOI: https://doi.org/10.3389/fragi.2021.797040
Journal volume & issue: Vol. 2

Abstract

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Human immune system functions over an entire lifetime, yet how and why the immune system becomes less effective with age are not well understood. Here, we characterize peripheral blood mononuclear cell transcriptome from 132 healthy adults with 21–90 years of age using the weighted gene correlation network analyses. In our study, 113 Caucasian from the 10KIP database and RNA-seq data of 19 Asian (Chinese) are used to explore the differential co-expression genes in PBMC aging. These two dataset reveal a set of insightful gene expression modules and representative gene biomarkers for human immune system aging from Asian and Caucasian ancestry, respectively. Among them, the aging-specific modules may show an age-related gene expression variation spike around early-seventies. In addition, we find the top hub genes including NUDT7, CLPB, OXNAD1, and MLLT3 are shared between Asian and Caucasian aging related modules and further validated in human PBMCs from different age groups. Overall, the impact of age and race on transcriptional variation elucidated from this study may provide insights into the transcriptional driver of immune aging.

Published in Frontiers in Aging

ISSN: 2673-6217 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Special situations and conditions: Geriatrics
Website: https://www.frontiersin.org/journals/aging#

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