Antibodies (Feb 2023)

Non-Affinity Purification of Antibodies

  • Tsutomu Arakawa,
  • Yui Tomioka,
  • Masataka Nakagawa,
  • Chiaki Sakuma,
  • Yasunori Kurosawa,
  • Daisuke Ejima,
  • Kouhei Tsumoto,
  • Teruo Akuta

DOI
https://doi.org/10.3390/antib12010015
Journal volume & issue
Vol. 12, no. 1
p. 15

Abstract

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Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.

Keywords